Phenotypic and molecular diversity among soybean cultivars as a function of growing season
Abstract
The identification of divergence among cultivars adapted to specific soil and climatic conditions is of fundamental importance for the realization of promising hybridizations. This diversity can be accessed through phenotypic characteristics and molecular markers. In this sense, the objective of this study was to evaluate genetic diversity of soybean cultivars in the summer and winter from agromorphological traits and molecular markers. Two experiments were conducted, one in the summer (2011) and another in the winter (2012). The experiments were conducted in a randomized block design with four replications. Six widely grown cultivars were used and they showed striking differences in regards to morphological markers. The cultivars are: M 7211 RR, TMG 123 RR, TMG 1176 RR, M 7908 RR, TMG 127 RR and TMG 7188 RR. During the development of the plant, as well after harvesting were evaluated 18 traits. The DNA of six cultivars was amplified with 16 primers (microsatellite markers) flanking microsatellite regions in soybean located in sixteen of the twenty soybean linkage groups. Genetic dissimilarity between cultivars from agromorphological traits varies depending on the growing season. The molecular markers showed genetic variability between cultivars with different results for clusters formed from the agronomic characters. Thus, both phenotypic and the molecular data proved to beinformative tools to characterize the existing conflict between soybean cultivars.
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References
Almeida RD, Peluzio JM and Afférri FS (2011) Divergência genética entre cultivares de soja, sob condições de várzea irrigada, no sul do Estado Tocantins. Revista Ciência Agronômica 42: 108-115.
Arriel NHC (2004) Diversidade genética em gergelim (Sesamum indicum L.) a partir de marcadores moleculares (RAPD) e caracteres morfológicos e agronômicos. Thesis, Universidade Estadual Paulista.
Bertini CHCM, Schuster I, Sediyama T, Barros EG and Moreira MA (2006) Characterization and genetic diversity analysis of cotton cultivars using microsatellites. Genetics and Molecular Biology 29: 321-329.
Bonato ALV, Calvo ES, Geraldi IO and Arias CAA (2006) Genetic similarity among soybean (Glycine max (L.) Merrill) cultivars released in Brazil using AFLP markers. Genetics and Molecular Biology 29: 692-704.
Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, Kaya N, Vantoai TT, Lohnes DG, Chung L and Specht JE (1999) An integrated genetic linkage map of the soybean genome. Crop Science 39: 1464-1590.
Cruz CD (2013) GENES - A software package for analysis in experimental statistics and quantitative genetics. Acta Scientiarum. Agronomy 35: 271-276.
Cruz CD and Carneiro PCS (2006) Modelos biométricos aplicados ao melhoramento genético. Editora UFV, Viçosa, v.2.
Cruz CD, Ferreira FM and Pessoni LA (2011) Biometria aplicada ao estudo da diversidade genética. Editora Suprema, Visconde do Rio Branco.
Dias LAS (1998) Análises multidimensionais. In: Alfenas AC (ed.) Eletroforese de isoenzimas e proteínas afins: fundamentos e aplicações em plantas e microorganismos. Editora UFV, Viçosa, p. 405-475.
Diwan N and Cregan PB (1997) Automated sizing fluorescent-labeled simple repeat marker to assay genetic variation in soybean. Theoretical and applied genetics 95: 723-731.
Fehr WR and Caviness CE (1977) Stage of soybean development. Iowa State University of Science and Technolog, Ames, 11p. (Special report 80).
Liu M, Zhang M, Jiang W, Sun G, Zhao H and Hu S (2011) Genetic diversity of Shaanxi soybean landraces based on agronomic traits and SSR markers. African Journal of Biotechnology 10: 4823-4837.
Meyer AS (2002) Comparação de coeficientes de similaridade usados em análises de agrupamentos com dados de marcadores moleculares dominantes. Dissertation, Universidade de São Paulo �?? Escola Superior de Agricultura Luiz de Queiroz.
Nelson RL and Li Z (1998) RAPD marker diversity among soybean and wild soybean accessions from four Chinese povinces. Agronomy Abstracts, ASA, Madison, p. 164.
Passianotto ALL (2010) Identificação molecular de cultivares de soja (Glycine max L. Merril) utilizando um sistema semi-automatizado de genotipagem. Dissertation, Universidade Estadual de Maringá.
Peluzio JM, Vaz-de-Melo A, Afférri FS, Silva RR, Barros HB, Nascimento IR and Fidelis RR (2009) Variabilidade genética entre cultivares de soja, sob diferentes condições edafoclimáticas. Pesquisa Aplicada & Agrotecnologia 2: 21-29.
Priolli RHG, Mendes-Junior CT, Sousa SMB, Sousa NEA and Contel EPB (2004). Diversidade genetica da soja entre periodos e entre programas de melhoramento no Brasil. Pesquisa agropecuaria brasileira 39: 967-975.
Priolli RHG, Pinheiro JB, Zucchi MI, Bajay MM and Vello, NA (2010). Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data. Brazilian Archives of Biology and Technology 53: 519-531.
Rigon JPG, Capuani S, Brito Neto JF, Rosa GM, Wastowski AD and Rigon CAG (2012) Dissimilaridade genética e análise de trilha de cultivares de soja avaliada por meio de descritores quantitativos. Revista Ceres 59: 233-240.
Rongwen J, Akkaya MS, Lavi U and Cregan PB (1995) The use of microsatellite DNA for soybean genotype identification. Theoretical and Applied Genetics 90: 43-48.
Santos ER, Barros HB, Ferraz EC, Cella AJS, Capone A, Santos AF and Fidelis RR (2011) Divergência entre genótipos de soja, cultivados em várzea irrigada. Revista Ceres 58: 755-764.
Schuster I, Queiroz VT, Teixeira AI, Barros, EG and Moreira MA (2004) Determinação da pureza varietal de sementes de soja com auxílio de marcadores moleculares microssatélites. Pesquisa Agropecuária Brasileira 39: 247-253.
Singh D (1981) The relative importance of characters affecting genetic divergence. The Indian Journal of Genetics e Plant Breeding 41: 237-245.
Singh SP, Gutiérrez JA, Molina A, Urrea C and Gepts P (1991) Genetic diversity in cultivated common beans: II.
Markers based analysis of morphological and agronomic traits. Madison. Crop Science 31: 23-29.
Song QJ, Marek, LF, Schoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE and Cregan PB (2004) A new integrated genetic linkage map of the soybean. Theoretical and Applied Genetic 109: 122-128.
Song QJ, Quicley CV, Nelson RL, Carter TE, Boerma HR, Stracha JR and Cregan PB (1999) A selected set of trinucleotide simple sequence repeat markers for soybean cultivar identification. Plant Varieties and Seeds 12: 207-20.
Soybase (2015) SoyBase and the Soybean Breeder�??s Toolbox. http://www.soybase.org/. Accessed 19 March 2015.
Vieira ESN, Schuster I, Silva RB and Oliveira MAR (2009) Variabilidade genética em cultivares de soja determinada com marcadores microssatélites em gel de agarose. Pesquisa Agropecuária Brasileira 44: 1460-1466.
Villela OT (2014) Diversidade fenotípica e molecular de cultivares brasileiras de soja portadoras de gene RR. Dissertation, Universidade Estadual Paulista.
doi.org/10.33158/ASB