Cryopreservation of seeds of Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k. by the vitrification method

  • Wagner Vendrame University of Florida https://orcid.org/0000-0001-6391-7623
  • Roberto Jun Takane Universidade Federal do Ceara https://orcid.org/0000-0002-3391-1826
  • Lamartine Soares Cardoso de Oliveira Universidade Federal do Ceara
  • Leonardo Alvarez Rios Universidade Nacional de Colômbia
  • Ricardo Tadeu de Faria Universidade Estadual de Londrina
Keywords: Conservation, germination, liquid nitrogen, liquid nutrient, organs, tissues

Abstract

The Brazilian Caatinga houses a number of cacti species, which are considered endangered due to human influence. Two of them, Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k., are endemic of the Brazilian Caatinga. Cryopreservation can provide a means for long-term preservation of endangered plant material. The aim of this study was to evaluate the efficiency of cryoprotectants for the cryopreservation of seeds from these two ­species. The treatments consisted of immersing seeds in different cryoprotectant solutions and vitrification solutions before storage in liquid nitrogen (NL) at -196 °C, as follows: T1- Control: no cryoprotectants; T2- PVS2 (10 min), T3-  PVS2 + phloroglucinol 1% (10 min), T4- PVS2 (10 min) + Supercool 1%; T5- PVS2 + phloroglucinol 1% (10 min) + Supercool 1%. Seed germination was evaluated after cryopreservation. The experimental design was completely randomized with five treatments and five replicates per treatment. Seed moisture at the beginning of the experiment was 6.2% for M. zehntneri and 7.8% for C. gounellei. There were no statistically significant differences for the seed germination percentage among the different treatments. Therefore, we concluded that seed of these two cacti species can be cryopreserved directly without the need for cryoprotectant solutions.

CROSSMARK_Color_horizontal.svg

Downloads

Download data is not yet available.

Author Biographies

Wagner Vendrame, University of Florida

Environmental Horticulture

Roberto Jun Takane, Universidade Federal do Ceara

Departamento de Fitotecnia

Lamartine Soares Cardoso de Oliveira, Universidade Federal do Ceara

Departamento de Fitotecnia

Ricardo Tadeu de Faria, Universidade Estadual de Londrina

Departamento de Fitotecnia

References

Bajaj, Y. P. S. (1995). Cryopreservation of plant cell, tissue, and organ culture for the conservation of germplasm and biodiversity . In Y. P. S. Bajaj (Ed.), Biotechnology in agriculture and forestry. (pp. 3�??28). Berlin: Springer.

Benson, E. E. (2008). Cryopreservation theory. Plant Cryopreservation: A Practical Guide, 15�??32. https://doi.org/10.1007/978-0-387-72276-4_2

Bewley, J. D., & Black, M. (1986). Seeds: physiology of development and germination. (2nd ed.). New York: Plenum Press.

Engelmann, F. (2011). Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular and Developmental Biology - Plant, 47(1), 5�??16. https://doi.org/10.1007/s11627-010-9327-2

Galdiano, R. F., Lemos, E. G. M., Faria, R. T., & Vendrame, W. A. (2012). Cryopreservation of Dendrobium hybrid seeds and protocorms as affected by phloroglucinol and Supercool X1000. Scientia Horticulturae, 148, 154�??160. https://doi.org/10.1016/j.scienta.2012.09.036

Hirano, T., Godo, T., Mii, M., & Ishikawa, K. (2005). Cryopreservation of immature seeds of Bletilla striata by vitrification. Plant Cell Reports, 23(8), 534�??539. https://doi.org/10.1007/s00299-004-0893-9

Hirano, Tomonari, Yukawa, T., Miyoshi, K., & Mii, M. (2011). Wide applicability of cryopreservation with vitrification method for seeds of some cymbidium species. Plant Biotechnology, 28(1), 99�??102. https://doi.org/10.5511/plantbiotechnology.10.1115a

LEE, J. M., & LOONEY, N. E. (1978). Changes in Abscisic Acid and Gibberellin Levels in Apple Seeds During Stratification and Their Relationship To Genetic Compaction. Canadian Journal of Plant Science, 58(3), 761�??767. https://doi.org/10.4141/cjps78-112

Lopes, K. P., Almeida, F. de A. C., Carvalho, J. M. F. C., & Bruno, R. de L. A. (2013). Criopreservação de eixos embrionários zigóticos de algodoeiro. Revista Brasileira de Engenharia Agrícola e Ambiental, 17(3), 291�??298. https://doi.org/10.1590/s1415-43662013000300007

Marcos Filho, J. (2005). Fisiologia de sementes de plantas cultivadas. Londrina, PR, Brasil: ABRATES.

Ministério da Agricultura, Pecuária e Abastecimento (MAPA). (2009). Regras para Análise de Sementes. Brasília: MAPA.

Nishizawa, S., Sakai, A., Amano, Y., & Matsuzawa, T. (1993). Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification. Plant Science, 91(1), 67�??73. https://doi.org/10.1016/0168-9452(93)90189-7

Pinfield, N.J., Stutchburry, P.A., & Bazaid, S. M. (1987). Seed dormancy in Acer: is there a common mechanism for all Acer species and what part is played in it by abscisic acid?, 71, 365�??371.

Powell, L. E. (1987). Hormonal aspects of bud and seed dormancy in temperature-zone woody plants. HortScience, 22(5), 845�??850.

Queiroz, L. P. (2006). Flowering plants of the Brazilian Semi-Arid. In A. M. Queiroz, L.P. Rapini, A. Giulietti (Ed.), Towards greater knowledge of the Brazilian Semi-Arid Biodiversity (pp. 45�??49). Brasília.: Ministério de Ciência e Tecnologia (MCT).

Rocha, M. S. (2009). Crioconservação de algodão colorido. Revista Brasileira de Engenharia Agrícola e Ambiental, 13(3), 312�??318.

Sakai, A., & Engelmann, F. (2007). Vitrification, encapsulation vitrification and droplet- vitrification: a review. CryoLetters, 28(3), 151�??172.

Sakai, A., Kobayashi, S., & Oiyama, I. (1990). Survival by Vitrification of Nucellar Cells of Navel Orange (Citrus sinensis var. brasiliensis Tanaka) by vitrification. Plant Cell Reports, 9, 30�??33. https://doi.org/10.1016/S0176-1617(11)80318-4

Santos, I. R. I. (2000). Criopreservação: potencial e perspectivas para a conservação de germoplasma vegetal. Revista Brasileira de Fisiologia Vegetal, 12, 70�??84.

Stanwood, P. C. (1980). Tolerance of crops seeds cooling and storage in liquid nitrogen. Journal of Seed Technology, 5(1), 26�??31.

Stanwood, P. C. (1985). Cryopreservation of seed germplasm for genetic conservation. In K. K. Kartha (Ed.), Cryopreservation of plant cells and organs (pp. 199�??255). Boca Raton: CRC Press.

Taylor, N.P., & Zappi, D.C. (2004). Cacti of eastern Brazi. Kew: Royal Botanic Gardens.

Thammasiri, K. (2000). Cryopreservation of seeds of a Thai orchid (Doritis pulcherrima Lindl.) by vitrification. CryoLetters, 21(4), 237�??244.

Veiga-Barbosa, L., González-Benito, M. E., Assis, J. G. A., & Pérez-García, F. (2010). Germination and cryopreservation of several cactus species from NE Brazil. Seed Science and Technology, 38(1), 218�??224. https://doi.org/10.15258/sst.2010.38.1.22

Vendrame, W. A., & Faria, R. T. (2011). Phloroglucinol enhances recovery and survival of cryopreserved Dendrobium nobile protocorms. Scientia Horticulturae, 128(2), 131�??135. https://doi.org/10.1016/j.scienta.2010.12.018

Published
2020-05-28
How to Cite
Vendrame, W., Takane, R., Cardoso, L., Alvarez, L., & Tadeu, R. (2020). Cryopreservation of seeds of Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k. by the vitrification method. Agronomy Science and Biotechnology, 6, 1-7. https://doi.org/10.33158/ASB.r106.v6.2020

Most read articles by the same author(s)