Effects of thermotherapy and meristem culture on forage production and nutrition value in elephant grass cultivars
Abstract
Elephant grass plays important role in dairy cattle production and has received special attention due to its potential in bioenergy and phytoremediation, as well as to its medicinal properties. The aim was investigating the effects of thermotherapy and meristem culture on elephant grass (Cenchrus purpureus (Schumach.) Morrone) forage production and nutritional value. Cultivars “Mineiro”, “Taiwan A-147” and “Pioneiro” were subjected to the methods: thermotherapy plus meristem culture, meristem culture and mature stems (control). The experiment assessed the tiller number (TN), tiller height (TH), number of leaves/tiller (NLT), leaf/stem ratio (LSR), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), at three cuts performed every 60 days for 180 days. It was observed beneficial influence on the evaluated traits, which indicated how cleaning methods work. Cultivar “Mineiro”, with more than 20 years of use, was more sensitive to the cleaning methods showing than cultivars “Taiwan A-147” (15 years of use) and “Pioneiro” (less than 10 years). It was observed that methods have affected the NT, TH, SDM, LSR, LDM, CP, and NDF. And, that the cultivars genotype effect in a different way the NT, TH, NLT, SDM, LSR, and CP. Overall, Cleaning methods produced an increase in the evaluated parameters over 100% for the cultivars Mineiro and Taiwan when compared with the traditional method and presented low or negative effect for Pioneiro cultivar This evidenced that the traditional propagation method affects forage production and quality over generations in vegetative propagated species. Basic biotechnology techniques such as meristem culture associated or not with thermotherapy can restore the productive potential being recommended for old asexually propagated cultivars with more than fifteen years of cultivation.
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Accosta, K., Pantoja, M.Q., & Perez-Lopez, E. Diversity of phytoplasma in Cuba, their geographic isolation and potential development of management strategies. In: Olivier, C., Dumonceaux, T., & Pérez-López, E. (Eds.). Sustainable Management of Phytoplasma Diseases in Crops Grown in the Tropical Belt. Sustainability in Plant and Crop Protection, vol 12. (p. 87-123). Cham: Springer. , https://doi.org/10.1007/978-3-030-29650-6_4
Anderlini, T. A., & Kostka, S. J. (1986). Initial yield responses of kleentek tissue culture-produced seedcane in Louisiana. Proceedings ISSCT, 19, 391–401.
Alves, F. G. S, Carneiro, M. S. S., Edvan, R.L., Pimentel, P.G., Ribeiro, M.X., ... & Vieira, M.C.C. (2020). Effect of cutting frequency on the yield and properties of elephant grass biomass for bioenergy and animal feed. International Journal of Agriculture and Natural Resources, 47(1), 12-22.
AOAC - Association of Official Agricultural Chemists. (1990). Official methods of analisys. 15th ed. v.1. Rockville, MD.
Asudi, G. O., Muyekho, F. N., Midega, C. A. O., & Khan, Z. R. (2019). Integrated Management of Napier Grass Stunt Disease in East Africa. In Olivier, C., Dumonceaux, T., & Pérez-López, E. (Eds.). Sustainable Management of Phytoplasma Diseases in Crops Grown in the Tropical Belt. Sustainability in Plant and Crop Protection, vol 12. (p. 105-123). Cham: Springer.. https://doi.org/10.1007/978-3-030-29650-6_5
Azeke, E. M., Eze, C. L., Ubong, I., & Kuroshi, L. (2019). The Potential of Elephant Grass (Pennisetum Purpureum Schum), a Nigerian Indigenous Grass, in Bioethanol Production: A Decarbonization Alternative for the Maritime Industry. Southern African Transport Conference. http://hdl.handle.net/2263/74305
Campos, B. B., Diniz, R. H., Silveira, F. A. D., Ribeiro, J. I., Fietto, L. G., Machado, J. C., & Silveira, W. B. D. (2019). Elephant grass (Pennisetum purpureum Schumach) is a promising feedstock for ethanol production by the thermotolerant yeast Kluyveromyces marxianus CCT 7735. Brazilian Journal of Chemical Engineering, 36, 43-49. https://doi.org/10.1590/0104-6632.20190361s20170263
Cha-um, Y., Hein, T. T., & Kirdmanee, C. (2006). Disease-free production of sugarcane varieties (Saccharum officinarum L.). Biotechnology, 5(4),443-448.
Cheong, E. J., Mock, R., & Li, R. (2012). Elimination of five viruses from sugarcane using in vitro culture of axillary buds and apical meristems. Plant Cell, Tissue and Organ Culture (PCTOC), 109(3), 439-445. https://doi.org/10.1007/s11240-011-0108-3
Crespo, G., & Álvarez, J. (2016). Comparison of biomass production of Pennisetum purpureum clones N fertilized. Cuban Journal of Agricultural Science, 48(3), 287-291.
Cronauer, S. S., & Krikorian, A. D. (1985). Aseptic multiplication of banana from excised floral apices. HortScience, 20(4), 770-771. https://doi.org/10.21273/HORTSCI.20.4.770
Deepthi, D. C., & Makeshkumar, T. (2016). Elimination of cassava mosaic disease through meristem culture and field evaluation for yield loss assessment in cassava genotypes. Journal of Root Crops, 42(1), 45-52. http://journal.isrc.in/index.php/jrc/article/view/397
Drew, R. A., & Smith, M. K. (1990). Field evaluation of tissue-cultured bananas in south-eastern Queensland. Australian Journal of Experimental Agriculture, 30(4), 569-574. https://doi.org/10.1071/EA9900569
Drew, R. A., & Vogler, J. N. (1993). Field evaluation of tissue-cultured papaw clones in Queensland. Australian Journal of Experimental Agriculture, 33(4), 475-479. https://doi.org/10.1071/EA9930475
Ferraris, R. (1978). The effect of photoperiod and temperature on the first crop and ratoon growth of Pennisetum purpureum Schum. Australian Journal of Agricultural Research, 29(5), 941-950. https://doi.org/10.1071/AR9780941
Habte, E., Muktar, M. S., Abdena, A., Hanson, J., Sartie, A. M., Negawo, A. T., ... & Jones, C. S. (2020). Forage performance and detection of marker trait associations with potential for Napier grass (Cenchrus purpureus) improvement. Agronomy, 10(4), 542. https://doi.org/10.3390/agronomy10040542
Herrera, R. S., García, M., Cruz, A. M., & Romero, A. (2012). Evaluación de clones de Pennisetum purpureum obtenidos por cultivo de tejidos in vitro. Revista Cubana de Ciencia Agrícola, 46(4), 427-433.
Herrera, R. S., Garcia, M., Fortes, D., Cruz, A. M., & Romero, A. (2013). Variability of the agronomic indicators of Pennisetum purpureum cv. Cuba CT-115 with the sampling distance. Cuban Journal of Agricultural Science, 47(3), 295-299.
Johns, G. G. (1994). Field evaluation of five clones of tissue-cultured bananas in northern NSW. Australian journal of experimental agriculture, 34(4), 521-528. https://doi.org/10.1071/EA9940521
Johnston, M., Onwueme, I. C., Dowling, A. J., & Rodoni, B. C. (1997). Comparison of suckering, leaf and corm characteristics of taro grown from tissue culture and conventional planting material. Australian journal of experimental agriculture, 37(4), 469-475. https://doi.org/10.1071/EA96059
Kartha, K. K., & Gamborg, O. L. (1975). Elimination of cassava mosaic disease by meristem culture. Phytopathology, 65(7), 826-828.
Karasawa, M. M. G., Pinto, J. E. B. P., Pinto, J. C., & Pereira, A. V. (2002). Proliferação de capim elefante em diferentes concentrações de regulador de crescimento e consistências do meio de cultura. Ciência e Agrotecnologia, 26(6), 1243-1251.
Karasawa, M. M. G., Pinto, J. C., Pereira, A. V., & Pinto, J. E. B. P. (2006). Avaliação da consistência do meio de cultura na propagação in vitro de capim-elefante (Pennisetum purpureum Schum.). Plant Cell & Micropropagation, 2(1), 35-42.
Karasawa, M. M. G., Pinto, J. E. B. P., Pereira, A. V., Pinto, J. C., & Silva, F. G. (2021). In: Vitro Propagation of Pennisetum purpureum Schum. International Grassland Congress. São Pedro: FEALQ. https://uknowledge.uky.edu/igc
Kassanis, B., & Varma, A. (1967). The production of virus‐free clones of some British potato varieties. Annals of Applied Biology, 59(3), 447- 450. https://doi.org/10.1111/j.1744-7348.1967.tb04461.x
Kawube, G., Talwana, H., Nicolaisen, M., Alicai, T., Otim, M., Kabirizi, J., ... & Nielsen, S. L. (2015). Napier grass stunt disease prevalence, incidence, severity and genetic variability of the associated phytoplasma in Uganda. Crop Protection, 75, 63-69. https://doi.org/10.1016/j.cropro.2015.04.020
Kogej, Z., Dermastia, M., & Mehle, N. (2020). Development and Validation of a new TaqMan real-time PCR for detection of ‘Candidatus Phytoplasma pruni’. Pathogens, 9(8), 642. https://doi.org/10.3390/pathogens9080642
Kumar, P. L., Cuervo, M., Kreuze, J. F., Muller, G., Kulkarni, G., Kumari, S. G., ... & Negawo, A. T. (2021).
Phytosanitary interventions for safe global germplasm exchange and the prevention of transboundary pest spread: the role of CGIAR germplasm health units. Plants, 10(2): 328. https://doi.org/10.3390/plants10020328
Lassois, L., Lepoivre, P., Swennen, R., Houwe, I. V. D., & Panis, B. (2012). Thermotherapy, chemotherapy, and meristem culture in banana. In: Protocols for micropropagation of selected economically-important horticultural plants (pp. 419-433). Totowa, NJ: Humana Press. https://doi.org/10.1007/978-1-62703-074-8_32
Li, X., Geng, X., Xie, R., Fu, L., Jiang, J., Gao, L., & Sun, J. (2016). The endophytic bacteria isolated from elephant grass (Pennisetum purpureum Schumach) promote plant growth and enhance salt tolerance of Hybrid Pennisetum. Biotechnology for Biofuels, 9(1), 1-12. https://doi.org/10.1186/s13068-016-0592-0
Lounglawan, P., Lounglawan, W., & Suksombat, W. (2014). Effect of cutting interval and cutting height on yield and chemical composition of King Napier grass (Pennisetum purpureum x Pennisetum americanum). APCBEE procedia, 8, 27-31. https://doi.org/10.1016/j.apcbee.2014.01.075
Lloyd, G.B., & McCown, B.H. (1980). Commercialy feasible micropropagation of moutain (Kalmia latifolia) by use of shoot tip culture. Combined Proceedings International Plant Propagators´ Society, 30, 421-437.
Macedo Júnior, G. L., Zanine, A. M., Borges, I., & Pérez, J. R. O. (2007). Qualidade da fibra para a dieta de ruminantes. Ciência Animal, 17(1), 7-17.
Marafon, A. C. C., Amaral, A. F. C., Machado, J. C., Carneiro, J., Bierhals, A. N., & Guimarães, V. D. S. (2020). Elephant grass biomass attributes for bioenergetic applications compared to other feedstocks. Available at SSRN 3598084. http://dx.doi.org/10.2139/ssrn.3598084
Mellor, F. C., & Stace-Smith, R. (1977). Virus-free potatoes by tissue culture. In: Reinert, J., Bajaj, Y.P.S. Applied and fundamental aspects of plant cell, tissue and organ culture (p.616-646). Berlin: Springer-Verlag.
Meng, J. Y., Ntambo, M. S., Luo, L. M., Huang, M. T., Fu, H. Y., & Gao, S. J. (2019). Molecular identification of Xanthomonas albilineans infecting elephant grass (Pennisetum purpureum) in China. Crop Protection, 124, 104853. https://doi.org/10.1016/j.cropro.2019.104853
Mohammed, I. Y., Abakr, Y. A., Kazi, F. K., Yusup, S., Alshareef, I., & Chin, S. A. (2015). Comprehensive characterization of Napier grass as a feedstock for thermochemical conversion. Energies, 8(5), 3403-3417. https://doi.org/10.3390/en8053403
Mora, B. V., & Silva, G. R. L. (1988). Efecto de un regulador del crescimiento en plantas sobre la producción del pasto elefante (Pennisetum purpureum). Veterinária México, 19, 225-229.
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Plant Physiology, 15(3), 473-497.
Negawo, A. T., Teshome, A., Kumar, A., Hanson, J., & Jones, C. S. (2017). Opportunities for Napier grass (Pennisetum purpureum) improvement using molecular genetics. Agronomy, 7(2), 28. https://doi.org/10.3390/agronomy7020028
Ocumpaugh, W. R., & Sollenberg, L. E. (1995). Other grasses for the humid South. In: Barnes, R. F., Miller, D. A., & Nelson, C. J. (Eds.). Forages. Ames, Iowa: Iowa State University Press.
Heather, O. V., & Hughes, H. M. (1980). The field performance of a clone of Cambridge Prizewinner strawberry, freed from latent virus A by meristem culture. Journal of Horticultural Science, 55(1), 79-82. https://doi.org/10.1080/00221589.1980.11514905
Panattoni, A., Luvisi, A., & Triolo, E. (2013). Elimination of viruses in plants: twenty years of progress. Spanish Journal of Agricultural Research, (1), 173-188.
Passos, L. P. (1999). Fisiologia do capim-elefante: uma revisão analítica. In: Passos, L.P., Carvalho, L.A., Martins, C.E., Bressan, M., Pereira, A.V. (Eds.). Biologia e manejo do capim-elefante. (p.29-62). Juiz de Fora: EMBRAPA/CNPGL.
Petersen, R. G. (1994). Agricultural field experiments: design and analysis. London: Crc Press.
Pereira, A. V., Ferreira, R. P., Passos, L. P., Freitas, V. P., Verneque, R. S., Barra, R. B., & Silva, C. H. P. (2000). Variation in leaf quality of elephant grass (Pennisetum purpureum) and elephant grass× pearl millet hybrids (P. purpureum× P. glaucum) as related to plant age. Ciência e Agrotecnologia, 24(2), 490-499.
Rodriguez, M. E., & Perez, E. (1987). Distribucion de carbono en un ecosistema de Panicum maximum, en ambiente modificado por el pastoreo. Ciencias, 59, 67.
Pierik, R. L. M. (1990). Cultivo in vitro de las plantas superiores. Madrid: Mundi-Prensa.
Quak, F. (1977). Meristem culture and virus-free plants. In: Reinert, J., & Bajaj, Y.P.S. (Eds.). Applied and fundamental aspects of plant cell, tissue and organ culture. (p. 130-140). Berlin: Springer Verlag.
Ramgareeb, S., Snyman, S. J., Antwerpen, T., & Rutherford, R. S. (2010). Elimination of virus and rapid propagation of disease-free sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture. Plant Cell, Tissue and Organ Culture (PCTOC), 100(2), 175-181. https://doi.org/10.1007/s11240-009-9634-7
Rocha, J. R. A. S. C., Machado, J. C., Carneiro, P. C. S., Carneiro, J. C., Resende, M. D. V., Lédo, F. J. S., & Carneiro, J. E. S. (2017). Bioenergetic potential and genetic diversity of elephantgrass via morpho-agronomic and biomass quality traits. Industrial Crops and Products, 95, 485-492. https://doi.org/10.1016/j.indcrop.2016.10.060
Santos, C. M., Nascimento, W. B. A., Nascimento, B. P., Schwab, S., Baldani, J. I., & Vidal, M. S. (2021). Temporal assessment of root and shoot colonization of elephant grass (Pennisetum purpureum Schum.) host seedlings by Gluconacetobacter diazotrophicus strain LP343. Microbiological Research, 244, 126651. https://doi.org/10.1016/j.micres.2020.126651
Samson, R., Mani, S., Boddey, R., Sokhansanj, S., Quesada, D., Urquiaga, S., ... & Ho Lem, C. (2005). The potential of C4 perennial grasses for developing a global BIOHEAT industry. Bpts, 24(5-6), 461-495. https://doi.org/10.1080/07352680500316508
Salvi, N. D., George, L., & Eapen, S. (2002). Micropropagation and field evaluation of micropropagated plants of turmeric. Plant Cell, Tissue and Organ Culture, 68(2), 143-151.
Santana, J. R., Pereira, J. M., Arruda, N. G., & Ruiz, M. A. M. (1989). Avaliação de cultivares de capim-elefante Pennisetum purpureum Schum. no sul da Bahia. I - Agrossistema cacaueiro. R Soc Bras Zootec, 18(3), 273-283.
Santos, M. C. S., Tabosa, J. N., Dias, F. M., Feitas, E. V., & Lira, M. A. (1994). Comportamento de clones de capim-elefante e de híbridos de capim-elefante x milheto no semi-árido do nordeste do Brasil1. Pesquisa Agropecuária Brasileira, 29(10), 1609-1615.
Silva, K. N., Nicolini, C., Silva, M. S., Fernandes, C. D., Nagata, T., & Resende, R. O. (2013). First Report of Johnsongrass mosaic virus (JGMV) Infecting Pennisetum purpureum in Brazil. Plant disease, 97(7), 1003. https://doi.org/10.1094/PDIS-01-13-0013-PDN
Smith, K. M. (1950). Virus de los vegetales. Buenos Aires: Acme Agency.
Smith, M. K., & Hamill, S. D. (1996). Field evaluation of micropropagated and conventionally propagated ginger in subtropical Queensland. Australian Journal of Experimental Agriculture, 36(3), 347-354. https://doi.org/10.1071/EA9960347
Tiwari, A. K., Tripathi, S., Lal, M., Sharma, M. L., & Chiemsombat, P. (2011). Elimination of sugarcane grassy shoot disease through apical meristem culture. Archives of Phytopathology and Plant Protection, 44(20), 1942-1948. https://doi.org/10.1080/03235408.2010.544446
Umami, N., Akashi, R., Gondo, T., Ishigaki, G., & Tanaka, H. (2016). Study on callus induction system of 4 genotype of Napiergrass (Pennisetum purpureum). Animal production, 18(3), 131-140. http://dx.doi.org/10.20884/1.anprod.2016.18.3.528
Uvidia, H., Ramírez, J., Vargas, J., Leonard, I., & Sucoshañay, J. (2015). Rendimiento y calidad del Pennisetum purpureum vc Maralfalfa en la Amazonía ecuatoriana. REDVET. Revista Electrónica de Veterinaria, 16(6), 1-11.
Videira, S. S., de Oliveira, D. M., de Morais, R. F., Borges, W. L., Baldani, V. L. D., & Baldani, J. I. (2012). Genetic diversity and plant growth promoting traits of diazotrophic bacteria isolated from two Pennisetum purpureum Schum. genotypes grown in the field. Plant and Soil, 356, 51-66. https://doi.org/10.1007/s11104-011-1082-6
Videira, S. S., Silva, M. C. P., Galisa, P. S., Dias, A. C. F., Nissinen, R., Divan, V. L. B., ... & Salles, J. F. (2013). Culture-independent molecular approaches reveal a mostly unknown high diversity of active nitrogen-fixing bacteria associated with Pennisetum purpureum—a bioenergy crop. Plant and soil, 373, 737-754. https://doi.org/10.1007/s11104-013-1828-4
Wang, P. J., & Charles, A. (1991). Micropropagation through meristem culture. In: High-Tech and Micropropagation I (p. 32-52). Berlin, Heidelberg: Springer.
Wilson, J. R., & Minson, D. J. (1980). Prospects for improving the digestibility and intake of tropical grasses. Tropical grasslands, 14(3), 253-259.
Zailan, M. Z., Yaakub, H., & Jusoh, S. (2018). Yield and nutritive quality of Napier (Pennisetum purpureum) cultivars as fresh and ensiled fodder. JAPS: Journal of Animal & Plant Sciences, 28(2).
Zanete, F., Pailo, W.N. & Moraes, A. (1988). Obtenção de mudas de capim-elefante (Pennisetum purpureum Schum. var. napier) por cultura de meristemas. Revista do Setor Ciências Agrárias, 10 (1-2), 175-177.
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