Characterization of Alternaria alternata causal agent of brown spot in Citrus spp

Aline Vanessa Sauer; Hugo José Tozze Júnior; Marcel Bellato Spósito; Eduardo Feichtenberger Feichtenberger; Nelson Barros Colauto; Luzia Doretto Paccola-Meirelles; Nelson Sidnei Massola Júnior

doi:10.33158/ASB.2015v1i2p45

Aline Vanessa Sauer Universidade Estadual de Londrina (UEL)
Hugo José Tozze Júnior Escola Superior de Agricultura Luiz de Queiroz - UESP
Marcel Bellato Spósito Fundecitrus, Departamento Científico
Eduardo Feichtenberger Feichtenberger Agência Paulista de Tecnologia dos Agronegócios - APTA
Nelson Barros Colauto Universidade Paranaense
Luzia Doretto Paccola-Meirelles Universidade Estadual de Londrina - UEL
Nelson Sidnei Massola Júnior Escola Superior de Agricultura Luiz de Queiroz USP

DOI: https://doi.org/10.33158/ASB.2015v1i2p45

Keywords: Variability, Enzymes production, Isoenzymes, Fungi, Citrus disease.

Abstract

The purpose of this study was to characterize culturally, enzymatically and pathogenically Alternaria alternata isolates obtained of tangerine/tangor (TP) and rough lemon (RLP). Significant differences were observed regarding mycelial growth speed and sporulation of isolates when cultivated in starch-agar (SA), potato-dextrose-agar (PDA) and tomato juice agar (V8) media. SA and PDA media promoted better mycelial growth and sporulation, respectively. Eight genetic similarity groups were defined through isoenzymatic characterization but without correlation between isolates and host or site of origin. All isolates produced amylase, cellulase, polygalacturonase and pectynase; however, no lipolytic or proteolytic activity was observed. Disease incubation period varied between 24 to 48 h for all isolates in all phenological stages of the inoculated fruit. Symptomatic fruit incidence in most tangerine and tangor isolates was higher in stage F3 compared to stages F4 and F5. Alternaria alternata have the ability to perform hyphal anastomosis indicating that this can be a mechanism used by the fungus to increase genetic variability.

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Author Biographies

Aline Vanessa Sauer, Universidade Estadual de Londrina (UEL)

Universidade Estadual de Londrina, Departamento de Agronomia, CP 6001, CEP 86051-990, Londrina, PR, Brasil.

Hugo José Tozze Júnior, Escola Superior de Agricultura Luiz de Queiroz - UESP

Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Departamento de Entomologia, Fitopatologia e Zoologia Agrícola, CP 09, CEP 13418-900, Piracicaba, SP, Brasil

Marcel Bellato Spósito, Fundecitrus, Departamento Científico

Fundecitrus, Departamento Científico, CP 391, CEP 14807-040, Araraquara, SP, Brasil

Eduardo Feichtenberger Feichtenberger, Agência Paulista de Tecnologia dos Agronegócios - APTA

APTA/SAA, Unidade de Pesquisa e Desenvolvimento de Sorocaba, CEP: 8013-440, Sorocaba, SP, Brasil.

Nelson Barros Colauto, Universidade Paranaense

Universidade Paranaense, Biotecnologia Aplicada à Agricultura, CEP 87502-210, Umuarama, PR, Brasil

Luzia Doretto Paccola-Meirelles, Universidade Estadual de Londrina - UEL

Universidade Estadual de Londrina, Departamento de Agronomia, CP 6001, CEP 86051-990, Londrina, PR, Brasil,

Nelson Sidnei Massola Júnior, Escola Superior de Agricultura Luiz de Queiroz USP

Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Departamento de Entomologia, Fitopatologia e Zoologia Agrícola, CP 09, CEP 13418-900, Piracicaba, SP, Brasil.

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Characterization of Alternaria alternata causal agent of brown spot in Citrus spp

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