Diversidade Genética de raças de Colletotrichum lindemuthianum baseadas nas regiões ITS do rDNA
Resumo
O Colletotrichum lindemuthianum é o agente causal da antracnose no feijão comum. Condições favoráveis �??�??para esta doença podem resultar em perdas de rendimento de até 100%. Um dos principais desafios para os produtores e melhoristas de feijão comum ainda continua sendo o manejo da doença, uma vez que, este patógeno, apresenta uma ampla variabilidade genética, provavelmente, devido à sua reprodução sexuada de recombinação. O gene 5.8S e as regiões espaçadoras internas transcritas flanqueadas (ITS1 e ITS2) de 40 diferentes isolados de C. lindemuthianum coletados no Brasil foram amplificados por PCR e sequenciados para determinar a variabilidade genética. Os resultados revelaram que 46,88% dos SNPs foram detectados na região ITS1, enquanto 53,12% deles estavam localizados na região ITS2. A distância genética variou de 0,000 a 0,169 entre as raças. A maior distância foi observada entre as raças 10 e 73 com valor de 0,169, indicando ampla variabilidade genética entre elas. A árvore filogenética foi composta por três grupos. O Grupo I apresentou cinco subgrupos. Resultados semelhantes também foram observados por meio da análise da estrutura populacional, que revelou a presença de três clusters. Esses resultados sugerem que a análise da sequência de regiões ITS do rDNA de C. lindemuthianum pode ser uma ferramenta valiosa para identificar este patógeno através do desenho de primers específicos.
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