Overexpression of full-length and partial DREB2A enhances soybean drought tolerance

Juliane Prela  Marinho; Renata Fuganti Pagliarini; Mayla Daiane Correa Molinari; Juliana Marcolino-Gomes; André Luís Hartmann  Caranhoto; Silvana Regina Rockenbach Marin; Maria Cristina Neves Oliveira; José Salvador Simonet Foloni; Carlos Lasaro Pereira Melo; Satoshi Kidokoro; Junya Mizoi; Norihito  Kanamori; Kazuko  Yamaguchi-Shinozaki; Kazuo  Nakashima; Alexandre Lima  Nepomuceno; Liliane Marcia Mertz-Henning

doi:10.33158/ASB.r141.v8.2022

Juliane Prela Marinho Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
Renata Fuganti Pagliarini Embrapa Soybean https://orcid.org/0000-0001-9282-2826
Mayla Daiane Correa Molinari Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0002-9135-0422
Juliana Marcolino-Gomes Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
André Luís Hartmann Caranhoto Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0003-3209-7962
Silvana Regina Rockenbach Marin Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
Maria Cristina Neves Oliveira Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
José Salvador Simonet Foloni Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
Carlos Lasaro Pereira Melo Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0003-4787-8382
Satoshi Kidokoro Laboratory of Plant Molecular Physiology. Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Junya Mizoi Plant Molecular Physiology Laboratory. Applied Biological Chemistry Department. University of Tokyo, Bunkyo-ku, Tokyo, Japan. https://orcid.org/0000-0002-3383-8059
Norihito Kanamori Division of Biological Resources and Post-harvest. Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan.
Kazuko Yamaguchi-Shinozaki Plant Molecular Physiology Laboratory. Applied Biological Chemistry Department. University of Tokyo, Bunkyo-ku, Tokyo, Japan
Kazuo Nakashima Division of Biological Resources and Post-harvest. Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan. https://orcid.org/0000-0001-6417-8316
Alexandre Lima Nepomuceno Embrapa Soybean, Carlos João Strass Road. Orlando Amaral Acess. Warta. PO. Box 231 86001-970, Londrina, PR, Brazil.
Liliane Marcia Mertz-Henning Embrapa Soybean, Carlos João Strass Road. Orlando Amaral Acess. Warta. PO. Box 231 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0001-7622-0649

DOI: https://doi.org/10.33158/ASB.r141.v8.2022

Resumo

Soybean is an important commodity worldwide. Abiotic conditions can adversely disturb crop growth and final yield. The transcription factor Dehydration-Responsive Element-Binding Proteins 2 (DREB2) act as a regulator of drought-responses. This study aimed to characterize soybean plants genetically modified with GmDREB2A;2 FL and GmDREB2A;2 CA for molecular, physiological, and agronomic responses, at different developmental periods. Results showed that seedlings from GmDREB2A;2 FL event presented lower growth reduction under osmotic treatment during germination. The GmDREB2A;2 FL and GmDREB2A;2 CA events showed improved performance in experiments of water deficit imposed in the vegetative period and higher rates in physiological parameters. In the reproductive period, there was a trend of higher yield compounds in GM GmDREB2A;2 FL event when compared to other genotypes and treatments. It was suggested that GmDREB2A;2 FL event presented superior performance due to the higher expression levels of the cisgene and drought-induced genes.

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