Substrates and indolbutyric acid in ex vitro rooting of blackberry and raspberry mini-cuttings
Resumo
The objective of this work was to determine the most adequate substrate and indolbutyric acid (IBA) concentration for the ex vitro rooting of the �??Xavante�?� and �??Tupy�?� blackberry and the �??Heritage�?� and �??Fall Gold�?� raspberry mini-cuttings. Explants were transferred to a laminar flow chamber and placed in a MS nutrient medium, giving origin to mini-cuttings whose bases immersed in four different IAC concentrations (0 mg.L-1, 1000 mg.L-1, 2000 mg.L-1, 3000 mg.L-1). Next, they were placed in transparent and articulated Sampack® (10 x 13 x 20) plastic bags with two types of substrates (vermiculite and vermiculite + coconut fiber, in the proportion of 1:1 v/v). For the number of roots variable, vermiculite substrate associated with coconut fiber showed representative results for the Tupy cultivar. However, for the Heritage cultivar, satisfactory results were obtained for the same variable with vermiculite substrate. One point of maximum efficiency was observed with the 1800 mg.L-1 IBA concentration. Results showed that the ex vitro rooting of blackberry and raspberry mini-cuttings may be realized without immersion in an IBA solution. Therefore, the use of vermiculite associated with coconut fibber is the most recommended.Downloads
Referências
Damiani CR and Schuch MW (2009) Diferentes substratos e ambientes no enraizamento in vitro de mirtilo. Ciência Rural 39(2): 563-566.
Feyissa T, Welander M and Negash L (2007) Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica. Biologia Plantarum 51 (1): 15-21.
Hartmann HT, Kester DE, Davies FT and Geneve RL (1997) Plant propagation: principles and practices. 6. ed. Prentice Hall, New Jersey, p.549-622.
Krikorian AD (1991) Medios de cultivo: generalidades, composición y preparación. In: Roca WR and Mroginski LA Cultivo de tejidos en la agricultura: fundamentos y aplicaciones. Colombia: Centro Internacional de Agricultura Tropical, Cali, p.41-78.
Leitzke LN, Damiani CR and Schuch MW (2009) Meio de cultura, concentração de AIB e tempo de cultivo no enraizamento in vitro de amoreira-preta e framboeseira. Revista Brasileira de Fruticultura 31(2): 582-587.
Leitzke LN, Damiani CR and Schuch MW (2010) Influência do meio de cultura, tipo e concentração de citocininas na multiplicação in vitro de amoreira-preta e framboeseira. Ciência e Agrotecnologia 34(2): 352-360.
Murashige T and Skoog F (1962) Revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15: 473-497.
Pelizza TR, Nascimento DC, Affonso LB, Camargo SS, Carra B and Schuch MW (2012) Enraizamento de plântulas de mirtileiro em condição ex vitro em diferentes substratos. Revista Brasileira de Fruticultura 34 (1):255-261.
Pelizza TR, Muniz J, Camargo P, Kretzschmar AA and Rufato L (2013) Enraizamento ex vitro e aclimatização de plântulas micropropagadas de amoreira-preta �??Xavante�??. Revista Brasileira de Fruticultura 35 (1): 333-337.
Reetz ER, Kist BB, Santos CE, Carvalho C and Drum M (2015) Anuário Brasileiro de Fruticultura 2015: brazilian fruit yearbook. Editora Gazeta, Santa Cruz do Sul, 108p.
