Physic nut germplasm genetic diversity identified by internal simple sequence repeats (ISSR) markers

Stella Maris Nucci; Maria Imaculada Zucchi; Glyn Mara Figueira; Renata Silva-Mann; José Baldin Pinheiro

doi:10.33158/ASB.2016v2i2p48

Stella Maris Nucci Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz
Maria Imaculada Zucchi Agência Paulista de Tecnologia dos Agronegócios
Glyn Mara Figueira Universidade Estadual de Campinas - UNICAMP
Renata Silva-Mann Universidade Federal de Sergipe - UFS
José Baldin Pinheiro Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz

DOI: https://doi.org/10.33158/ASB.2016v2i2p48

Resumo

The physic nut has attracted great interest for their socioeconomic promising vegetable oil production capacity that can be used in biodiesel manufacturing. However, the species is still under a domestication process and many studies are still needed to start a breeding program. In this context, this study aimed to evaluate the genetic diversity in 29 physic nut accessions from two germplasm banks using 14 ISSR markers. Genetic characterization revealed that 86.64% of the genetic variation is present within the groups, whereas 13.36% corresponded to the variation between the groups. The total mean number of alleles per locus was 1.99 and the effective number of alleles corresponded to 1.42. The Bayesian analysis allocated the access into four clusters, showing that the clusters formed showed no relation to the origin of access. The Jaccard similarity index indicated that the extent of similarity between accessions ranged from 0.23 to 0.94. The dendrogram formed the same four access groups as those formed by the Bayesian analysis, and all groups were in agreement in both analyses. The study demonstrated the need and importance of a germplasm collection from several regions and countries in order to preserve the genetic diversity of the species and the development of Euphorbiaceae breeding programs

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Biografia do Autor

Stella Maris Nucci, Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz

Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, Caixa Postal 83, CEP 13400-970 Piracicaba, SP, Brazil.

Maria Imaculada Zucchi, Agência Paulista de Tecnologia dos Agronegócios

Agência Paulista de Tecnologia dos Agronegócios, Pólo APTA Regional Centro Sul, Caixa Postal 28, CEP 13400-970, Piracicaba, SP, Brazil.

Glyn Mara Figueira, Universidade Estadual de Campinas - UNICAMP

Universidade Estadual de Campinas, Centro Pluridisciplinar de Pesquisas Químicas e Biológicas, Caixa-Postal 6171, Paulínia, SP, Brazil.

Renata Silva-Mann, Universidade Federal de Sergipe - UFS

Universidade Federal de Sergipe, Centro de Ciências Biológicas e da Saúde, Departamento de Engenharia Agronômica, Av. Marechal Rondon s/n, CEP 49100-000, São Cristovão, SE, Brazil

José Baldin Pinheiro, Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz

Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, Caixa Postal 83, CEP 13400-970 Piracicaba, SP, Brazil.

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