Glyphosate impact on C and N microbial functional groups in soybean rhizosphere
Abstract
The no-tillage system requires a higher use of herbicides to control weeds before sowing, increasing the concern over the effect that such products might have on the environment. However, few studies reporting the influence of glyphosate on soil microorganisms are available in the literature. The aim of this work was to evaluate the effect of two formulations of glyphosate on functional groups of microorganisms in soybean rhizosphere. The experiment was performed in a greenhouse, and the treatments were the isopropylamine salt and the ammonium salt of glyphosate at the maximum and twice the maximum allowed dose. The control treatment had no application. The populations of soil bacteria, fungi, actinomycetes, fluorescent pseudomonas, cellulolytics, amylolytics, proteolytics and free-living N-fixing were determined by plate counts on selective media. Plant root dry weight, shoot dry weight and the number and dry weight of nodules were determined. The application of herbicides had little effect on the populations of microorganisms, as well as on plant growth. Nevertheless, the interactions among some populations of microorganisms and between these and the plant were influenced by the formulation of the glyphosate applied.Downloads
References
Abbas Z, Akmal M, Khan KS and Hassan F (2014) Effect of buctril super (Bromoxynil) herbicide on soil microbial biomass and bacterial population. Brazilian Archives of Biology and Technology 57: 9-14.
Andrade G (2004) Role of functional groups of microorganisms on the rhizosphere microcosm dynamics. In: Varma A, Abbott L, Werner D and Hampp R (eds.) Plant Surface Microbiology. Springer-Verlag, Berlin, p. 51-69.
Chagas Junior AF, Reis MR, Santos GR, Erasmo EAL and Chagas LFB (2013) Nodulation and mycorrhization of transgenic soybean after glyphosate application. Semina: Ciências Agrárias 34: 3675-3682.
Cely MVT, Siviero MA, Emiliano J, Spago FR, Freitas VF, Barazetti AR, Goya ET, Lamberti GS, dos Santos IMO, de Oliveira AG and Andrade G (2016) Inoculant of Schizolobium parahyba with mycorrhizal fungi and plant growth-promoting rizobacteria increases wood yield under field conditions. Frontiers in Plant Science 7: 1708.
Duke OS, Lydon J, Koskinen WC, Moorman TB, Chaney RL and Hammerschmidt R (2012) Glyphosate effects on plant mineral nutrition, crop rhizosphere microbiota, and plant disease in glyphosate-resistant crops. Journal of Agricultural and Food Chemistry 60: 10375-10397.
Fehr WR and Caviness CE (1977) Stages of soybean development. Iowa State University, Special Report 80.
Gimsing ALG, Borggaard OK, Jacobsen OS, Aamand J and Sørensen J (2004) Chemical and microbiological soil characteristisc controlling glyphosate mineralisation in Danish surface soils. Applied Soil Ecology 27: 233-242.
Hiltner L (1904) Uber neuere Erfahrungen und Probleme auf dem Gebiete der Bodenbakteriologie unter besonderden berucksichtigung und Brache. Arbeiten der Deutschen Landwirtschaftlichen Gesellschaft 98: 59-78.
Kremer RJ and Means NE (2009) Glyphosate and glyphosate-resistant crop interactions with rhizosphere microorganisms. European Journal of Agronomy 31: 153-161.
Maeda H and Dudareva N (2012) The shikimate pathway and aromatic amino acid biosynthesis in plants. Annual Review of Plant Biology 63: 73-105.
Martinez-Toledo MV, Salmeron V and Gonzalez-Lopez J (1990) Effect of phenoxy and benzoic acid herbicides on nitrogenase activity and growth of Azospirillum brasilense. Soil Biology and Biochemistry 22: 879-881.
Mekonnen G and Dessie M (2016) Effect of pre-emergence herbicides on weeds, nodulation and yield of cowpea [Vigna unguiculata (L.) WALP.] at Haik and Mersa in Wollo, northern Ethiopia. International Journal of Applied Agricultural Sciences 2: 99-111.
Mertens M, Hoss S, Neumann G, Afzal J and Reichenbecher (2017) Glyphosate, a chelating agent-relevant for ecological risk assessment?. Environmental Science and Pollution Research. doi: 10.1007/s11356-017-1080-1.
Nye M, Hoilett N, Ramsier C, Renz P and Dick RP (2014) Microbial community structure in soils amended with glyphosate-tolerant soybean residue. Applied Ecology and Environmental Sciences 2: 74-81.
Ramos MAG and Yoshioka SA (2012) Bioremediation of herbicide Velpar K® in vitro in aqueous solution with application of EM-4 (effective microorganisms). Brazil Archives of Biology and Technology 55: 145-149.
Ratcliff AW, Busse MD and Shestak CJ (2006) Changes in microbial community structure following herbicide (glyphosate) additions to forest soils. Applied Soil Ecology 34: 114-124.
Powles SB and Yu Q (2010) Evolution in action: Plants resistant to herbicides. Annual Review of Plant Biology 61: 317-347.
Singh BK, Walker A, Morgan JAW and Wright DJ (2004) Biodegradation of chlorpyrifos by enterobacter strain b-14 and its use in bioremediation of contaminated soils. Applied and Environmental Microbiology 70: 4855-4863.
Smith P, Cotrufo MF, Rumpel C, Paustian K, Kuikman PJ, Elliot JA, McDowell R, Griffiths RI, Asakawa S, Bustamante M, House JI, Sobocká J, Harper R, Pan G, West PC, Gerber KS, Clark JM, Adhya T, Scholes RJ and Scholes MC (2015) Biogeochemical cycles and biodiversity as key drivers of ecosystem services provided by soils. Soil 1: 665-685.
Sviridov AV, Shushkova TV, Ermakova IT, Ivanova EV, Epiktetov DO and Leontievsky AA (2015) Microbial degradation of glyphosate herbicides (review). Applied Biochemistry and Microbiology 51: 188-195.
Trezzi MM, Kruse ND and Vidal RA (2001) Inibidores de EPSPS. In: Vidal RA and Merotto A Jr (eds.) Herbicidologia. Evangraf, Porto Alegre, p. 37-45.
Varanasi VK, Bayramov S, Prasad PVV and Jugulam M (2017) Expression profiles of psba, ALS, ESPS, and other chloroplastic genes in response to PSII-ALS, and EPSPS-inhibitor treatments in Kochia scoparia. American Journal of Plant Sciences 8: 451-470.
Zabaloy MC, Zanini GP, Bianchinotti V, Gomez MA and Garland JL (2011) Herbicides in the soil environment: linkage between bioavailability and microbial ecology. In: Soloneski S and Larramendy ML (eds.) Herbicides, Theory and Applications. InTech, Rijeka, p. 161-192.
Zuberer DA (1994) Recovery and enumeration of viable bacteria. In: Weaver RW, Angle JS, and Bottomley PS (eds.) Methods of Soil Analysis, Part 2, Microbiological and biochemical properties. Soil Science Society of Agronomy, Madison, p. 119-144.
doi.org/10.33158/ASB