Survival of pantoea ananatis, causal agent of maize white spot disease in crop debris

  • Aline Vanessa Sauer Universidade Estadual de Londrina (UEL)
  • Katia Regina Rocha Universidade Estadual de Londrina (UEL)
  • Ricardo Martins Gonçalves Universidade Estadual de Londrina (UEL)
  • Walter Fernandes Meirelles Embrapa Milho e Sorgo
  • José Edson Fontes Figueiredo Embrapa Milho e Sorgo
  • Ivanildo Evódio Marriel Universidade Estadual de Londrina (UEL)
  • Luzia Doretto Paccola-Meirelles Universidade Estadual de Londrina (UEL)
Keywords: Epiphytic bacteria, Digitaria horizontalis, Metabolic fingerprint, Ice-nucleation, Corn crop residues

Abstract

Maize white spot, caused by the bacterium Pantoea ananatis, is one of the most detrimental diseases of maize in Brazil and has contributed to significant yield reduction. In this study, P. ananatis isolates were recovered from maize white spot lesions, healthy leaves, corn crop residues and Digitaria horizontalis. All the bacterial isolates were identified and characterized by morphological traits analysis, acid production from sorbitol and glycerol, ice nucleation activity, metabolic fingerprint similarities and molecular analysis by Polymerase Chain Reaction with species-specific primers for P. ananatis (ANAF/ANAR). Results showed that isolates recovered from maize white spot lesions were similar to those isolates collected from other sources. It suggests that corn crop residues, D. horizontalis and healthy leaf surface of the corn plant itself, could be a source of survival for P. ananatis.

CROSSMARK_Color_horizontal.svg

Downloads

Download data is not yet available.

Author Biographies

Aline Vanessa Sauer, Universidade Estadual de Londrina (UEL)
Universidade Estadual de Londrina, Londrina, PR, Brazil
Katia Regina Rocha, Universidade Estadual de Londrina (UEL)
Universidade Estadual de Londrina, Londrina, PR, Brazil
Ricardo Martins Gonçalves, Universidade Estadual de Londrina (UEL)
Universidade Estadual de Londrina, Londrina, PR, Brazil
Walter Fernandes Meirelles, Embrapa Milho e Sorgo
Embrapa Milho e Sorgo, Sete Lagoas, MG, Brazil
José Edson Fontes Figueiredo, Embrapa Milho e Sorgo
Embrapa Milho e Sorgo, Sete Lagoas, MG, Brazil
Ivanildo Evódio Marriel, Universidade Estadual de Londrina (UEL)
Universidade Estadual de Londrina, Londrina, PR, Brazil
Luzia Doretto Paccola-Meirelles, Universidade Estadual de Londrina (UEL)
Universidade Estadual de Londrina, Londrina, PR, Brazil

References

Alippi AM and López AC (2010) First report of leaf spot disease of maize caused by Pantoea ananatis in Argentina. Plant Disease 94: 487.

Cota LV, Costa RV, Silva DD, Parreira DF, Lana UGP and Case¬la, CR (2010) First report of pathogenicity of Pantoea ananatis in sorghum (Sorghum bicolor) in Brazil. Australasian Plant Disease Notes 5: 120-122.

Cother EJ, Noblea DH, van de Vena RJ, Lanoiseletb V, Ashd G, Vuthyc N, Visartoc P and Stodartd B (2010) Bacterial pathogens of rice in the Kingdom of Cambodia and description of a new pathogen causing a serious sheath rot disease. Plant Pathology 59: 944 �??953.

Coutinho TA and Venter SN (2009) Pantoea ananatis: an uncon¬ventional plant pathogen. Molecular Plant Pathology 10: 325- 335.

Escanferla ME, Wysmierski PT, Meirelles WF and Pacco¬la-Meirelles LD (2006) Crescimento, viabilidade e disseminação da bactéria Pantoea ananatis, agente causal da doença Mancha Branca do Milho. In: Congresso Nacional de Milho e Sorgo, 26, 2006; Simpósio Brasileiro sobre a Lagarta do Cartucho, 2; Simpósio sobre Colletotrichum Graminicola,1, 2006. Belo Hori¬zonte. Anais�?� Belo Horizonte. CD-ROM.

FAO (2013). http://www.fao.org. Accessed 04 Nov. 2013.

Figueiredo JEF and Paccola-Meirelles LD (2012) Simple, rapid and accurate PCR-based detection of Pantoea ananatis in maize, sorghum and Digitaria sp. Journal of Plant Pathology 94: 663- 667.

Gitaitis R, Walcott R, Culpepper S, Sanders H, Zolobowska L and Langston D (2002) Recovery of Pantoea ananatis, causal agent of center rot of onion, from weeds and crops in Georgia, USA. Crop Protection 21: 983-989.

Goszczynska T, Botha WJ, Venter SN and Coutinho TA (2007) Isolation and identification of the causal agent of brown stalk rot, a new disease of maize in South Africa. Plant Disease 91: 711-718.

Gürtler V and Stanisich VA (1996) New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 142: 3-16.

Kido K, Adachi R, Hasegawa M, Yano K, Hikichi Y, Takeuchi

S, Atsuchi T and Takikawa Y (2008) Internal fruit rot of net¬ted melon caused by Pantoea ananatis (Erwinia ananas) in Japan. Journal of General Plant Pathology 74: 302-312.

Krawczyk K, Kamasa J, Zwolinska A and Pospieszny H (2010) First report of Pantoea ananatis associated with leaf spot disease of maize in Poland. Journal of Plant Pathology 92: 807-811.

Lindow SE, Arny DC and Upper CD (1978) Erwinia herbicola: a bacterial ice nucleus active in increasing frost injury to corn. Phytopathology 68: 523-527.

Malagi G, Santos I, Camochena RC and Moccellin R (2011) Diagrammatic scale for severity evaluation of maize white foliar spot. Revista Ciência Agronômica 42: 797-804.

Paccola-Meirelles LD, Ferreira AS, Meirelles WF, Marriel IE and Casela CR (2001) Detection of a bacterium associated with a leaf spot disease of maize in Brazil. Journal of Phytopathology 149: 275-279.

Pérez-Y-Terrón R, Villegas MC, Cuellar A, Muñoz-Rojas J, Castañeda-Lucio M, Hernández-Lucas I, Bustillos-Cristales R, Bautista-Sosa L, Munive JA, Caicedo-Rivas R and Fuen¬tes-Ramírez LE (2009) Detection of Pantoea ananatis, causal agent of leaf spot disease of maize, in Mexico. Australasian Plant Disease Notes 4: 96�??99.

Pinto NFJA (2004) Controle químico de doenças foliares em milho. Revista Brasileira de Milho e Sorgo 3: 134-138.

Romeiro RS (2001) Atividade de nucleação de gelo (INA). In: Romeiro RS (Ed.) Métodos em bacteriologia de plantas. Viçosa. UFV. pp. 209-211.

Schaad NW, Jones JB and Chun W (2001) Laboratory guide for identification of plant pathogenic bacteria. Biologia Plantarum 4: 546.

Sauer AV, Baba VY, Pedro ES, Meirelles WF and Paccola-Meire¬lles LD (2009) Monitoramento da população epifítica de Pan¬toea ananatis agente causal da mancha branca do milho In: XLlI Congresso Brasileiro de Fitopatologia, 2009, Rio de Janeiro- RJ. Tropical Plant Pathology 34: S1 - S370.

Walcott RR, Gitaitis RD, Castro AC, Sanders FH. Jr and Di¬az-Perez JC (2002) Natural infestation of onion seed by Pantoea ananatis, causal agent of center rot. Plant Disease 86: 106-111.

Wilson M, Hirano SS and Lindow SE (1999) Location and sur¬vival of leaf-associated bacteria in relation to pathogenicity and potential for growth within the leaf. Applied and Environmental Microbiology 65: 1435�??144324

Published
2015-06-23
How to Cite
Sauer, A. V., Rocha, K. R., Gonçalves, R. M., Meirelles, W. F., Figueiredo, J. E. F., Marriel, I. E., & Paccola-Meirelles, L. D. (2015). Survival of pantoea ananatis, causal agent of maize white spot disease in crop debris. Agronomy Science and Biotechnology, 1(1), 21. https://doi.org/10.33158/ASB.2015v1i1p21
Section
Article

Most read articles by the same author(s)